Steroids
Everything You Need to Know About Steroid Testing
Many bodybuilders today currently use steroids. Reasons why vary anywhere from helping with muscle growth to more drive in the weight room. No matter what side of the fence you’re on over athletes’ and bodybuilders’ use of steroids, if you plan on competing in any major bodybuilding or body sport competition, chances are you’re going to be tested for steroids.
Sports and steroid testing have gone hand in hand for nearly the last century. Testing came into play after steroids were banned because athletes began pushing their bodies too far and would habitually overdose. Over the years as steroids have become more advanced so have methods of testing.
What is steroid testing used for?
Steroid testing is used in most cases to determine an athlete’s eligibility to compete, as any trace of an enhancement can disqualify an athlete. Enhancements are a concern for many sports associations because they are known to give an unfair advantage over other competitors with no enhancement drugs.
How is a steroid test performed?
Steroid tests are done by taking a bodily fluid sample from the testee and analyzing it by altering the sample with other chemicals to detect any synthetic substances. With many injections or foreign compounds put into the body it can take several weeks to be detectable in the system, but enhancement drugs are not only designed to become effective immediately but also last in the system for long periods of time. This makes them detectable in a drug screen almost instantly after use and even up to several weeks afterwards.
Types of test samples:
Steroid testing can use bodily fluids for testing like urine and blood, and they can even detect steroid use from a follicle of hair.
How exactly are steroids detected?
To determine if an athlete has steroids in their system there’s a series of tests in place. The first (and for minor sports or leagues the only test) is what’s known as a T/E test. A T/E test looks for two different hormones from a urine sample, Testosterone and Epitestosterone. Testosterone is the more commonly familiar to the public of the two, which is simulated by steroids to produce bigger, stronger muscles and more physical drive. Epitestosterone in not commonly simulated by steroids.
These two hormones are produced naturally in the body in relatively equal amounts. But when steroids enter the system there is a much higher amount of testosterone present than epitestosterone. Steroids tests allow anywhere from 4:1 to 6:1 ratios of testosterone over epitestosterone to account for those who may possibly produce a high amount of testosterone naturally.
Now because there is a possibility to fail a T/E test even if you haven’t used steroids, it’s not typically used as the end all be all and is often just used as a screening to weed out the red flag athletes who need to be tested further. The official test is an isotope test which checks the ratios of carbon 12 to carbon 13 in the body. This test is considered relatively impossible to fake and is often the test that put the nail in the coffin for most professional athlete’s steroids scandals.
Humans naturally consist of both carbon 12 and carbon 13, and at a similar level much like T/E levels. However, because steroids are synthetically made by pharmaceutical corporations they don’t react naturally or contain the same levels of carbon that your body does. So steroids tend to contain slightly more carbon 13 than is naturally produced by the body, making the carbon 12 to 13 levels in the system unbalanced. This unbalance is what is detected by the isotope test.
With such advanced testing how are steroids tests faked?
Steroids are prevalent in virtually all sports, even at top professional levels where they are strictly prohibited. So it’s common to wonder how so many athletes are still able to pull it off even when they’re subjected to so much testing.
In most cases the only way an athlete is able to fake a drug test is on the screening levels where the tests are less advanced and not as precise. Because these tests are only used to spot the larger red flags that need to be investigated further this is often where the most tests are faked. The most commonly faked test is the T/E test, which is done by raising the levels of epitestosterone in the system. By taking synthetic epitestosterone, even though it won’t enhance sports performance, one will be able to even out their T/E ratios; making high testosterone levels appear normal and allowing the athlete to fly under the radar.
Are steroids tests harmful?
Steroid testing is not at all physically harmful to an athlete. However, at top levels one failed test can negatively damage the reputation of any athlete; this is why results are often kept secret as just an investigation alone (even without any steroids in play) can stigmatize athletes.
Though they aren’t physically harmful there has been a lot of controversy over whether testing is harmful to fans. Testing for steroids comes down to very advanced science which can be incredibly costly; this cost is reflected in ticket prices to games and events. As tests get more and more costly so does the burden on sports leagues need to offset this cost on its fans. Many argue that because of this, such advanced testing may not be worth it as sports leagues want us to think.
Steroids have long been the target of a frenzied witch hunt in sports, and unfortunately so. It’s no doubt that too much of anything is harmful to the system, but steroids have been proven to safely enhance the performance of our athletes when administered responsibly. Athletes and bodybuilders should be encouraged to reach maximum potential as they are trained professional. Allowing them to showcase their peak physical performance however they choose would not only produce more amazing athletes with amazing records but create incredible competition across the board.
Steroids
Decoding IGF-1 LR3: A Guide to its Benefits

IGF-1 LR3 (Insulin-like Growth Factor-1 Long Arg3) is a synthetic variant of IGF-1, a hormone produced by the liver in response to growth hormone (GH). Unlike GH, which works indirectly, IGF-1 directly facilitates the growth and repair of muscle cells.
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This modified version of IGF-1 is engineered to avoid binding with IGF-binding proteins, extending its half-life to 20–30 hours. As a result, it remains active in the body significantly longer than natural IGF-1.
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Many performance athletes incorporate IGF-1 LR3 post-workout to enhance muscle growth, speed up recovery, and support fat loss. When injected into specific muscle groups, it often produces localized effects. Additionally, it helps with nutrient distribution and, in some cases, improves insulin sensitivity.
On the other hand, human growth hormone (HGH) operates more broadly and indirectly, stimulating the liver to produce IGF-1 and offering more systemic benefits such as improved skin health, joint support, and fat reduction, albeit at a slower pace.
Typical IGF-1 LR3 doses range from 20–50 mcg daily, administered subcutaneously or intramuscularly, often following a workout.
Key Features of IGF-1 LR3
Enhanced Bioavailability: It avoids binding with IGF-binding proteins, increasing its potency.
Muscle Growth & Repair: Directly stimulates muscle cell proliferation and differentiation.
Improved Nutrient Distribution: Helps shuttle nutrients into muscle cells, supporting recovery.
Potential Fat Loss Benefits: Some users report improved insulin sensitivity and fat metabolism.
Mechanism of Action
IGF-1 LR3 interacts with IGF-1 receptors on muscle cells, activating pathways like PI3K-AKT and MAPK, which are crucial for cell growth and regeneration. This makes it a powerful tool for bodybuilders and athletes looking to optimize recovery and muscle development.
What Are the Top 10 Alternatives to IGF-1 LR3?
Here are some of the top alternatives to IGF-1 LR3, each with unique benefits for muscle growth, recovery, and performance:
BPC-157 – Known for its regenerative properties, it enhances healing and tissue repair.
CJC-1295 – A growth hormone-releasing peptide that boosts GH levels for muscle development.
Ipamorelin – Stimulates GH release with minimal side effects, making it a safer option.
MK-677 (Ibutamoren) – A potent GH secretagogue that promotes muscle growth and fat loss.
Sermorelin – Encourages natural GH production, supporting recovery and lean muscle gains.
Tesamorelin – Primarily used for fat loss, but also aids in muscle preservation.
Follistatin-344 – Inhibits myostatin, allowing for increased muscle hypertrophy.
PEG-MGF (Pegylated Mechano Growth Factor) – Enhances muscle repair and growth post-exercise.
GHRP-6 – Stimulates appetite and GH release, supporting muscle mass gains.
GHRP-2 – Similar to GHRP-6 but with fewer hunger-related side effects.
Each of these peptides has distinct mechanisms and benefits.
Must Read: Are Nootropics a Better Option to AAS?
Potential Risks Associated With IGF-1 LR3
IGF-1 LR3 comes with several potential risks, especially for bodybuilders using it to enhance muscle growth. Here are some key concerns:
Hypoglycemia (Low Blood Sugar) – IGF-1 LR3 increases glucose uptake in muscle cells, which can lead to dangerously low blood sugar levels if not managed properly.
Organ Growth – Since IGF-1 affects all tissues, excessive use may lead to unwanted growth in organs, increasing health risks.
Cancer Risk – IGF-1 plays a role in cell proliferation, and elevated levels have been linked to an increased risk of certain cancers.
Water Retention & Edema – Some users experience bloating and fluid retention, which can affect performance and aesthetics.
Joint Pain & Stiffness – Excessive IGF-1 levels may contribute to joint discomfort due to increased tissue growth.
Cardiovascular Issues – There is some concern that IGF-1 LR3 could contribute to heart enlargement or other cardiovascular complications.
Desensitization – Long-term use may reduce the body's natural IGF-1 production, leading to dependency
Overall
IGF-1 LR3's ability to bypass IGF-binding proteins makes it more potent but also increases the likelihood of desensitization with prolonged use. For those considering it, careful dosing and monitoring are crucial to mitigate side effects.
Steroids
AOD-9604: The Fat-Burning Peptide Explained

AOD-9604, along with the similar HGH Frag 176-191, is a peptide derived from Growth Hormone that includes only the amino acids in HGH responsible for stimulating fat breakdown, known as lipolysis.
This means that these peptides offer the fat-burning benefits of HGH without its other effects—whether beneficial or adverse—and come at a lower cost.
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Lipolysis refers to the process where fat cells are broken down to be utilized as energy. AOD-9604 promotes accelerated fat loss by increasing the body's use of fat as fuel.
For optimal results, the peptides should be used in a fasted state. AOD-9604 and Frag 176-191 are most effective when administered at a dose of 125-250mcg before bedtime (at least 3-4 hours after eating) and in the morning at the same dose, followed by a fasting period of 3-4 hours, ideally combined with fasted cardio.
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Similar Peptides with Fat-Burning Effect
Here’s a list of 10 peptides similar to AOD-9604, each with a brief description:
Ipamorelin: A growth hormone-releasing peptide (GHRP) that stimulates the natural release of growth hormone, promoting fat loss, muscle growth, and improved recovery without affecting other hormones like cortisol or prolactin.
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CJC-1295: A peptide that increases growth hormone and IGF-1 levels, aiding in fat loss, muscle gain, and improved sleep quality. It has a long half-life, making it convenient for users.
HGH Frag 176-191: A fragment of human growth hormone specifically designed for fat-burning. It targets adipose tissue without the broader effects of full-length HGH.
Tesamorelin: Known for reducing visceral fat, this peptide stimulates the release of growth hormone and is often used for weight management and metabolic health.
BPC-157: While primarily known for healing and recovery, BPC-157 can support fat loss indirectly by improving gut health and reducing inflammation.
Melanotan II: Originally developed for skin tanning, it also has appetite-suppressing properties, making it useful for weight management.
Thymosin Beta-4 (TB-500): Focused on healing and recovery, it can enhance physical performance and indirectly support fat loss through improved activity levels.
GHRP-6: A growth hormone-releasing peptide that boosts appetite and metabolism, aiding in muscle growth and fat loss.
Semaglutide: A GLP-1 receptor agonist that regulates appetite and blood sugar levels, making it effective for weight loss and metabolic health.
MK-677 (Ibutamoren): A growth hormone secretagogue that increases growth hormone and IGF-1 levels, promoting fat loss, muscle gain, and improved recovery.
List of Peptides With a Counteractive Effect Bodybuilders Must Avoid
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Some peptides can inadvertently lead to fat gain due to their effects on metabolism and appetite regulation. Here are a few that bodybuilders might want to avoid or use cautiously:
- GHRP-6 – While it stimulates growth hormone release, it also significantly increases appetite, which can lead to excess calorie consumption and fat gain.
- IGF-1 LR3 – This peptide enhances muscle growth but can also promote fat storage if not carefully managed with diet and training.
- MK-677 (Ibutamoren) – Though technically a growth hormone secretagogue rather than a peptide, it boosts GH levels but often leads to increased hunger and potential fat accumulation.
- CJC-1295 with DAC – While effective for muscle growth, its prolonged GH release can sometimes lead to unwanted fat retention if not paired with a strict diet
Overall
AOD-9604, derived from Growth Hormone, stimulates fat-burning by targeting lipolysis—the breakdown of fat for energy. It delivers these benefits without the additional effects of Growth Hormone, making it more cost-effective. For maximum effectiveness, it should be administered in a fasted state, ideally before bed and again in the morning at 125-250mcg doses. Pairing its use with fasting and fasted cardio can further enhance fat loss. Its sole focus is on improving the body’s ability to burn fat efficiently.
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Bodybuilding
Understanding Trenbolone-Induced Cough (“Tren Cough”)

Trenbolone, a potent anabolic steroid, can sometimes cause “tren cough”—a sudden and intense coughing episode shortly after injection. Although not exclusive to Trenbolone, it is more commonly associated with this substance due to its highly irritant nature.
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Mechanisms Behind Tren Cough
Solvent and Carrier Irritation
Trenbolone formulations often include volatile solvents like benzyl alcohol or benzyl benzoate, which may irritate lung tissues when absorbed quickly into systemic circulation.
Prostaglandin Release
Trenbolone promotes increased production of prostaglandins, particularly PGF2α. This compound triggers contraction in the smooth muscles of the lungs, leading to bronchoconstriction and coughing.
Micro-Oil Embolism
Tiny oil droplets from an injection can reach capillaries and travel to the lungs, causing mild embolic reactions that lead to temporary oxygen deprivation and coughing.
Histamine and Mast Cell Activation
For some individuals, Trenbolone triggers histamine release and mast cell activation, mimicking an allergic response and causing bronchospasms and cough reflexes.
Related Article: Best Syringes for Steroid Injection on Amazon
Using Salbutamol (Albuterol) to Manage Tren Cough
- Salbutamol, a widely-used β2-adrenergic receptor agonist, can alleviate tren cough symptoms by:
- Relaxing bronchial muscles, easing spasms that cause coughing.
- Inhibiting prostaglandin effects, reducing bronchoconstriction associated with PGF2α.
- Opening airways, preventing severe respiratory restrictions in susceptible individuals.
Application Methods
Inhaler (Optimal)
Take 1–2 puffs of salbutamol (100–200 mcg) 5–10 minutes before a Trenbolone injection. If coughing occurs afterward, additional puffs can swiftly resolve the issue.
Oral Tablets (Moderate)
Consuming 2–4 mg tablets 30–60 minutes before injection offers slower, longer-lasting relief but may be less effective than inhalation methods.
Nebulizer (Severe Cases)
For individuals with frequent episodes, nebulized doses of 2.5 mg salbutamol can provide substantial relief.
Preventive Measures to Reduce Tren Cough Risk
- Inject slowly to minimize systemic absorption and irritant effects.
- Split doses to lower reaction severity with smaller quantities.
- Opt for ventrogluteal injection sites, which have fewer blood vessels, reducing oil embolism risk.
- Choose lower-concentration solutions to lessen irritation, as higher concentrations (e.g., Tren Ace 200 mg/ml) are more likely to provoke reactions.
What Other Steroids Can Induce Coughing?
Here's a curated list of peptides, SARMs, and PEDs that may potentially cause coughing or respiratory irritation in bodybuilders and fitness enthusiasts:
Peptides
IGF-1 LR3 (Insulin-like Growth Factor): Known for its anabolic effects, IGF-1 LR3 can occasionally cause mild respiratory irritation due to systemic absorption.
TB-500 (Thymosin Beta-4): While rare, improper injection techniques or high doses may lead to transient coughing episodes.
GHRP-6 (Growth Hormone-Releasing Peptide): This peptide can stimulate histamine release, potentially leading to bronchospasms and coughing.
SARMs (Selective Androgen Receptor Modulators)
RAD-140: (Testolone) Some users report throat irritation or coughing, often attributed to solvents used in liquid formulations.
YK-11: Known for its myostatin-inhibiting properties, YK-11 may cause mild respiratory discomfort in sensitive individuals.
LGD-4033 (Ligandrol): Though uncommon, some users experience coughing due to carrier solvents or allergic-like reactions.
PEDs (Performance-Enhancing Drugs)
Boldenone Undecylenate (Equipoise): This injectable steroid can cause “Equipoise cough,” similar to tren cough, due to oil embolism or irritant solvents.
Testosterone Suspension: The water-based formulation may lead to coughing episodes if injected improperly or absorbed rapidly.
Nandrolone Decanoate (Deca-Durabolin): While less common, coughing can occur due to histamine release or systemic irritation.
More Tips to Minimize Coughing Risks
- Use proper injection techniques to avoid embolic reactions.
- Opt for lower-concentration solutions to reduce irritant effects.
- Consider antihistamines or bronchodilators for individuals prone to respiratory sensitivity.
Read More: Joint Stiffness: How to Manage It While on AAS
Are There Alternatives to Cough-inducing Steroids?
Here are some alternatives to cough-inducing steroids that can provide similar anabolic effects while minimizing respiratory irritation:
Peptides
IGF-1 LR3 (Insulin-like Growth Factor)
Promotes muscle growth and recovery without the irritant properties of certain steroids.
TB-500 (Thymosin Beta-4)
Enhances tissue repair and reduces inflammation, making it a safer option for recovery.
BPC-157 (Body Protection Compound)
Known for its healing properties, it supports muscle repair and joint health.
SARMs (Selective Androgen Receptor Modulators)
RAD-140 (Testolone)
Provides significant muscle-building effects with fewer systemic side effects compared to traditional steroids.
LGD-4033 (Ligandrol)
Boosts lean muscle mass and strength without the risk of respiratory irritation.
MK-677 (Ibutamoren)
Stimulates growth hormone release, aiding in muscle growth and recovery.
Natural Alternatives
Turkesterone
A plant-based ecdysteroid that supports muscle protein synthesis and recovery.
Ecdysterone
Another natural compound that mimics anabolic effects without the harsh side effects.
Creatine Monohydrate
Enhances strength and muscle mass through improved energy production during workouts.
Other Options
Human Growth Hormone (HGH)
Promotes muscle growth and fat loss, though it requires careful monitoring due to potential side effects.
Testosterone Boosters
Natural supplements like D-Aspartic Acid or Tribulus Terrestris can help optimize testosterone levels for muscle growth.
SARMs Alternatives
Legal and safer versions of SARMs are available, offering similar benefits without the risks associated with traditional SARMs.
Overall
We have explored the phenomenon of "tren cough," a sudden, intense coughing episode often caused by Trenbolone injections due to factors like solvent irritation, prostaglandin release, micro-oil embolism, or histamine activation. Preventive measures such as using salbutamol (via inhaler, oral tablets, or nebulizer), injecting slowly, splitting doses, and opting for lower-concentration solutions were highlighted.
Additionally, alternative compounds to tren cough-inducing steroids were discussed, including peptides like IGF-1 LR3 and TB-500, SARMs such as RAD-140 and LGD-4033, and natural options like Turkesterone, ecdysterone, and creatine. These alternatives provide anabolic effects while minimizing respiratory side effects. The conversation also underscored the importance of proper injection techniques and thoughtful compound selection to reduce risks.
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